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STEMCELL Technologies Inc methocult h4100
Methocult H4100, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methocult+h4100/methocult/pm40362524-350-40-45
Average 90 stars, based on 1 article reviews
methocult h4100 - by Bioz Stars, 2026-09
90/100 stars

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Centrifugation:

Article Title: Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions.
Article Snippet: .. The cells were then counted and diluted to 1 × 106 cells/mL based on the total volume needed (100 μL per well) before centrifugation at 1000 RPM for 5 min. Spheroid formation media were made in a 3:1 ratio of cell culture media/MethoCult H4100 (Cat#04100, StemCell Technologies, Cambridge, MA, USA), and the total volume was calculated at 100 μL per well. .. Cell pellets were resuspended in spheroid formation media and plated 100 μL per well in a round bottom low-attached 96-well plate (Cat#7007, Corning).

Article Title: Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions
Article Snippet: .. The cells were then counted and diluted to 1 × 10 6 cells/mL based on the total volume needed (100 μL per well) before centrifugation at 1000 RPM for 5 min. Spheroid formation media were made in a 3:1 ratio of cell culture media/MethoCult H4100 (Cat#04100, StemCell Technologies, Cambridge, MA, USA), and the total volume was calculated at 100 μL per well. .. Cell pellets were resuspended in spheroid formation media and plated 100 μL per well in a round bottom low-attached 96-well plate (Cat#7007, Corning).

Cell Culture:

Article Title: Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions.
Article Snippet: .. The cells were then counted and diluted to 1 × 106 cells/mL based on the total volume needed (100 μL per well) before centrifugation at 1000 RPM for 5 min. Spheroid formation media were made in a 3:1 ratio of cell culture media/MethoCult H4100 (Cat#04100, StemCell Technologies, Cambridge, MA, USA), and the total volume was calculated at 100 μL per well. .. Cell pellets were resuspended in spheroid formation media and plated 100 μL per well in a round bottom low-attached 96-well plate (Cat#7007, Corning).

Article Title: Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions
Article Snippet: .. The cells were then counted and diluted to 1 × 10 6 cells/mL based on the total volume needed (100 μL per well) before centrifugation at 1000 RPM for 5 min. Spheroid formation media were made in a 3:1 ratio of cell culture media/MethoCult H4100 (Cat#04100, StemCell Technologies, Cambridge, MA, USA), and the total volume was calculated at 100 μL per well. .. Cell pellets were resuspended in spheroid formation media and plated 100 μL per well in a round bottom low-attached 96-well plate (Cat#7007, Corning).

other:

Article Title: Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1
Article Snippet: For cell line experiments cells were seeded at 1000 cells/mL in methocult H4100 (StemCell Technologies) prepared at a 1:1:3 ratio of methocult:FCS:IMDM.

Article Title: The C-terminal PHDVC5HCH tandem domain of NSD2 is a combinatorial reader of unmodified H3K4 and tri-methylated H3K27 that regulates transcription of cell adhesion genes in multiple myeloma.
Article Snippet: Cells were grown in MethoCult H4100 (Stem Cell Technologies) supplemented with 20% FBS, 1%Pen Strep (Life Technologies) and 1%GlutaMax-I (Life Technologies).

Article Title: A potent and selective ENL degrader suppresses oncogenic gene expression and leukemia progression
Article Snippet: Colony formation experiments were performed in Methocult (STEMCELL Technologies, catalog no. H4100).

Article Title: Gadd45g insufficiency drives the pathogenesis of myeloproliferative neoplasms
Article Snippet: HEL and SET-2 cells were plated in MethoCult H4100 (Stemcell, Vancouver, BC, Canada), and colonies were counted on day 14 post plating.



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Image Search Results


Journal: iScience

Article Title: Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1

doi: 10.1016/j.isci.2025.112151

Figure Lengend Snippet:

Article Snippet: MethoCult H4100 (methylcellulose medium) , STEMCELL Technologies , Cat# 04100.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Functional Assay, Western Blot, Recombinant, Protease Inhibitor, Diagnostic Assay, Staining, Software